The Nucleosome Remodelling and Deacetylation complex restricts Mediator access to enhancers to control transcription
نویسندگان
چکیده
1. Wellcome Trust – Medical Research Council Stem Cell Institute, University of Cambridge, Gleeson Building, Tennis Court Road, Cambridge CB2 1QR United Kingdom 2. European Bioinformatics Institute, European Molecular Biology Laboratory (EMBL), Wellcome Trust Genome Campus, Cambridge CB10 1SD, United Kingdom 3. Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge CB2 1QR United Kingdom
منابع مشابه
Acetylation increases access of remodelling complexes to their nucleosome targets to enhance initiation of V(D)J recombination
Targeted chromatin remodelling is essential for many nuclear processes, including the regulation of V(D)J recombination. ATP-dependent nucleosome remodelling complexes are important players in this process whose activity must be tightly regulated. We show here that histone acetylation regulates nucleosome remodelling complex activity to boost RAG cutting during the initiation of V(D)J recombina...
متن کاملMBD3 Localizes at Promoters, Gene Bodies and Enhancers of Active Genes
The Mi-2/nucleosome remodeling and histone deacetylase (NuRD) complex is a multiprotein machine proposed to regulate chromatin structure by nucleosome remodeling and histone deacetylation activities. Recent reports describing localization of NuRD provide new insights that question previous models on NuRD action, but are not in complete agreement. Here, we provide location analysis of endogenous...
متن کاملTramtrack69 interacts with the dMi-2 subunit of the Drosophila NuRD chromatin remodelling complex.
dMi-2, the ATPase subunit of the Drosophila nucleosome remodelling and histone deacetylation (dNuRD) complex, was identified in a two-hybrid screen as an interacting partner of the transcriptional repressor, Tramtrack69 (Ttk69). A short region of Ttk69 is sufficient to mediate this interaction. Ttk69, but not the Ttk88 isoform, co-purifies with the dNuRD complex isolated from embryo extracts. d...
متن کاملSall4 controls differentiation of pluripotent cells independently of the Nucleosome Remodelling and Deacetylation (NuRD) complex
Sall4 is an essential transcription factor for early mammalian development and is frequently overexpressed in cancer. Although it is reported to play an important role in embryonic stem cell (ESC) self-renewal, whether it is an essential pluripotency factor has been disputed. Here, we show that Sall4 is dispensable for mouse ESC pluripotency. Sall4 is an enhancer-binding protein that prevents p...
متن کاملEpigenetic regulation in chondrogenesis.
Epigenetics is an essential mechanism to control gene expression and fundamental cellular processes. DNA methylation in CpG-rich promoters correlates with gene silencing. Histone modification including histone acetylation and deacetylation determines the stability of the chromatin structure. Condensed chromatin (heterochromatin), which has a higher-order histone-DNA structure, prevents the acce...
متن کامل